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The cells were consistently cultured in complete medium for 48 h, then the Beckman Coulter MoFlo Astrios Mobile Sorter was employed to pick out cells with CXCR4-damaging expression. These selected cells have been cultured in total medium supplemented with 1 μg/mL of puromycin.垂頭喪氣 �?潦�?的差別在哪裡�?備受影響 �?受到�